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DYNAMICS OF BENZO A PYRENE DIOL EPOXIDE ADDUCTS IN POLY(DG-DC) . (DG-DC) STUDIED BY SYNCHROTRON EXCITED FLUORESCENCE POLARIZATION ANISOTROPY DECAY

A. Graslund ; Seog K. Kim (Institutionen för fysikalisk kemi) ; Svante Eriksson (Institutionen för fysikalisk kemi) ; Bengt Nordén (Institutionen för fysikalisk kemi) ; B. Jernstrom
Biophysical Chemistry (0301-4622). Vol. 44 (1992), 1, p. 21-28.
[Artikel, refereegranskad vetenskaplig]

Time-resolved fluorescence studies have been performed on (+)-anti-7,8-dihydrodiol-9,10-epoxy-benzo[a]pyrene adducts in double-stranded poly(dG-dC) . (dG-dC). Part of the adduct population gives rise to excimer fluorescence. The heterogeneous fluorescence emission decay curves at 22-degrees-C could be resolved into three components with lifetimes: 0.4 ns, 3 ns and 24 ns for the total fluorescence (monomer and excimer emission), and 0.5 ns, 5 ns and 24 ns, respectively, for excimer emission alone. The relative amplitudes for the longer lifetimes were larger for the pure excimer population than for the mixed population. The fluorescence polarization anisotropy decay curves were resolved into two components of rotational correlation times: 0.4 ns and 25 ns for the total fluorescence and 0.3 ns and 33 ns for the excimer fluorescence. We interpret the two rotational correlation times to correspond to local motion of the adduct and segmental motion of the polynucleotide, respectively.

Nyckelord: benzo[a]pyrene diol epoxide, poly(dg-dc).(dg-dc), synchrotron radiation, fluorescence lifetimes, anisotropy decay, molecular-dynamics, dna, binding



Denna post skapades 2011-08-22.
CPL Pubid: 144645

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

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Molekylärbiologi

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