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Phospholipid membrane permeability of peptide nucleic acid

Pernilla Wittung (Institutionen för fysikalisk kemi) ; Johan Kajanus (Institutionen för organisk kemi) ; K. Edwards ; P. Nielsen ; Bengt Nordén (Institutionen för fysikalisk kemi) ; B. G. Malmstrom
Febs Letters (0014-5793). Vol. 365 (1995), 1, p. 27-29.
[Artikel, refereegranskad vetenskaplig]

Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone, The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.

Nyckelord: liposome, peptide nucleic acid, cryogenic transmission electron, microscopy, fluorescence, oligonucleotide analogs, dna, recognition, antisense, vesicles, thymine, pna

Denna post skapades 2011-08-17.
CPL Pubid: 144430


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Institutionen för fysikalisk kemi (1900-2003)
Institutionen för organisk kemi (1900-2003)



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