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METHYL GREEN - A DNA MAJOR-GROOVE BINDING-DRUG

Seog K. Kim (Institutionen för fysikalisk kemi) ; Bengt Nordén (Institutionen för fysikalisk kemi)
Febs Letters (0014-5793). Vol. 315 (1993), 1, p. 61-64.
[Artikel, refereegranskad vetenskaplig]

Interaction and binding geometries of complexes of Methyl green with poly(dA-dT)2, poly(dA) . poly(dT), and triplex poly(dA) . 2poly(dT) complexes have been studied by linear dichroism. For both of the complexes with double helical DNAs, the z symmetry axis of Methyl green is found to be approximately parallel to the DNA bases while the x symmetry axis lies at 40-44-degrees relative to the local DNA helix axis, in agreement with a groove binding mode. However, in contrast to minor-groove binders (such as DAPI and Hoechst 33258) Methyl green is found to be excluded from binding to the triple helical poly(dA) . 2poly(dT) in which the major groove is filled by the third strand. While most so far studied groove-binding dyes bind in the minor groove of DNA, Methyl green thus appears to be an exception.

Nyckelord: methyl green, triplex, major groove binding, dna, poly(da).poly(dt), poly(da).2poly(dt), linear dichroism, poly(dg-dc)



Denna post skapades 2011-08-17.
CPL Pubid: 144389

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Molekylärbiologi

Chalmers infrastruktur