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ENHANCEMENT OF BINDING RATE OF RECA PROTEIN TO DNA BY CARCINOGENIC BENZO A PYRENE DERIVATIVES AND SELECTIVE CHANGE OF ADDUCT CONFORMATION

Seog K. Kim (Institutionen för fysikalisk kemi) ; Masayuki Takahashi (Institutionen för fysikalisk kemi) ; B. Jernstrom ; Bengt Nordén (Institutionen för fysikalisk kemi)
Carcinogenesis (0143-3334). Vol. 14 (1993), 2, p. 311-313.
[Artikel, refereegranskad vetenskaplig]

The association kinetics of RecA protein from Escherichia coli to DNA is strongly enhanced if even a minor fraction of DNA bases has been modified by a carcinogenic (+)-anti metabolite of benzo[a]pyrene (BPDE). The enhancement is much smaller with the less carcinogenic (-)-anti enantiomer of BPDE suggesting the possibility that the RecA protein binds selectively to the proto-oncogenic target. Most importantly, the binding of RecA to DNA modified with the latter enantiomer is found to give rise to a reorganization of this BPDE adduct from an intercalation site into a minor groove site. This indicates that the binding mechanism of RecA is via intercalation of some amino acid moiety, a discovery that could explain the approximately 50% contour length increase of the DNA within its fibrous complex with RecA.

Nyckelord: linear dichroism spectroscopy, electron-microscopy, complexes, coordination



Denna post skapades 2011-08-16.
CPL Pubid: 144368

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Cancer och onkologi

Chalmers infrastruktur