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PNA HYBRIDIZES TO COMPLEMENTARY OLIGONUCLEOTIDES OBEYING THE WATSON-CRICK HYDROGEN-BONDING RULES

M. Egholm ; O. Buchardt ; L. Christensen ; C. Behrens ; S. M. Freier ; D. A. Driver ; R. H. Berg ; Seog K. Kim (Institutionen för fysikalisk kemi) ; Bengt Nordén (Institutionen för fysikalisk kemi) ; P. E. Nielsen
Nature (0028-0836). Vol. 365 (1993), 6446, p. 566-568.
[Artikel, refereegranskad vetenskaplig]

DNA ANALOGUES are currently being intensely investigated owing to their potential as gene-targeted drugs1-3. Furthermore, their properties and interaction with DNA and RNA could provide a better understanding of the structural features of natural DNA that determine its unique chemical, biological and genetic properties3,4. We recently designed a DNA analogue, PNA, in which the backbone is structurally homomorphous with the deoxyribose backbone and consists of N-(2-aminoethyl)glycine units to which the nucleobases are attached5-9. We showed that PNA oligomers containing solely thymine and cytosine can hybridize to complementary oligonucleotides, presumably by forming Watson-Crick-Hoogsteen (PNA)2-DNA triplexes, which are much more stable than the corresponding DNA-DNA duplexes5-7, and bind to double-stranded DNA by strand displacement5,8. We report here that PNA containing all four natural nucleobases hybridizes to complementary oligonucleotides obeying the Watson-Crick base-pairing rules, and thus is a true DNA mimic in terms of base-pair recognition.

Nyckelord: peptide nucleic-acids, dna, recognition, replication, antisense, stability, thymine, helices



Denna post skapades 2011-08-16.
CPL Pubid: 144347

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Kemi

Chalmers infrastruktur