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In vitro digestive stability of complexes between gliadin and synthetic blocking peptides

Karolina Hoffmann (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Nils-Gunnar Carlsson (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Marie Alminger (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Tingsu Chen ; Agnes E Wold ; Olof Olsson ; Ann-Sofie Sandberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap)
Biotechnology and Applied Biochemistry (0885-4513). Vol. 58 (2011), 3, p. 190-197.
[Artikel, refereegranskad vetenskaplig]

Celiac disease is caused by an inappropriate immune response to incompletely digested gluten proteins. We investigated whether synthetic peptides with high affinity to wheat gliadin could be selected with a phage display technique and whether complexes between such peptides and gliadin could sustain gastric and pancreatic digestion. Two synthetic peptides, P61 and P64, were selected because of their high affinity to immobilized gliadin. They were allowed to form complexes with gliadin, whereafter the complexes were subjected to in vitro digestion with gastric and pancreatic enzymes. The digestion products were analyzed with Western blot and RP HPLC. The results showed that both peptides formed stable complexes with intact gliadin and that complexes between gliadin and peptide P64 partly resisted gastrointestinal digestion. The two peptides reduced the binding of serum anti-gliadin IgA antibodies by 12%, and 11.5%, respectively, and the binding of anti-gliadin antibodies of the IgG isotype by 13% and 10%. Thus peptides produced by a phage display technique could interact stably with gliadin partly masking epitopes for antibody binding. A combination of peptides of this kind may be used to block gliadin-immune system interactions.

Nyckelord: celiac disease, gliadin, blocking peptides, in vitro digestion, RP, HPLC/immunochemistry, celiac-disease, allergens, proteins, cells



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Denna post skapades 2011-08-11. Senast ändrad 2015-09-04.
CPL Pubid: 144090

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)
Institutionen för cell- och molekylärbiologi (1994-2011)
Institutionen för biomedicin, avdelningen för infektionssjukdomar (GU)

Ämnesområden

Livsvetenskaper
Biokemi
Molekylärbiologi
Immunteknik
Immunologi inom det medicinska området

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