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STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION

M. Leijon ; A. Graslund ; P. E. Nielsen ; O. Buchardt ; Bengt Nordén (Institutionen för fysikalisk kemi) ; S. M. Kristensen ; M. Eriksson
Biochemistry (0006-2960). Vol. 33 (1994), 33, p. 9820-9825.
[Artikel, refereegranskad vetenskaplig]

The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

Nyckelord: thymine-substituted polyamide, nuclear magnetic-resonance, strand-displacement, coupling-constants, proton resonances, spectroscopy, suppression, oligonucleotides, assignment, spectra



Denna post skapades 2011-08-10.
CPL Pubid: 144033

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Molekylärbiologi

Chalmers infrastruktur