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The Antennapedia peptide penetratin translocates across lipid bilayers - the first direct observation

Per Thorén (Institutionen för fysikalisk kemi) ; Daniel Persson (Institutionen för fysikalisk kemi) ; M. Karlsson ; Bengt Nordén (Institutionen för fysikalisk kemi)
Febs Letters (0014-5793). Vol. 482 (2000), 3, p. 265-268.
[Artikel, refereegranskad vetenskaplig]

The potential use of polypeptides and oligonucleotides for therapeutical purposes has been questioned because of their inherently poor cellular uptake. However, the 16-mer oligopeptide penetratin, derived from the homeodomain of Antennapedia, has been reported to enter cells readily via a non-endocytotic and receptor- and transporter-independent pathway, even when conjugated to large hydrophilic molecules. We here present the first study where penetratin is shown to traverse a pure lipid bilayer. The results support the idea that the uptake mechanism involves only the interaction of the peptide with the membrane lipids. Furthermore, we conclude that the translocation does not involve pore formation.

Nyckelord: antennapedia homeodomain, penetratin, membrane translocation, delivery vector, uptake



Denna post skapades 2011-06-20.
CPL Pubid: 142023

 

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Institutioner (Chalmers)

Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Biokemi

Chalmers infrastruktur