CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Metal nanoparticles amplify photodynamic effect on skin cells in vitro

B. Bauer ; Si Chen (Institutionen för teknisk fysik, Bionanofotonik) ; Mikael Käll (Institutionen för teknisk fysik, Bionanofotonik) ; Linda Gunnarsson (Institutionen för teknisk fysik, Bionanofotonik) ; M. B. Ericson
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Optical Interactions with Tissue and Cells XXII; San Francisco, CA; 24-26 January 2011 (1605-7422). Vol. 7897 (2011),
[Konferensbidrag, övrigt]

We report on an investigation aimed to increase the efficiency of photodynamic therapy (PDT) through the influence of localized surface plasmon resonances (LSPR's) in metal nanoparticles. PDT is based on photosensitizers that generate singlet oxygen at the tumour site upon exposure to visible light. Although PDT is a well-established treatment for skin cancer, a major drawback is the low quantum yield for singlet-oxygen production. This motivates the development of novel methods that enhance singlet oxygen generation during treatment. In this context, we study the photodynamic effect on cultured human skin cells in the presence or absence of gold nanoparticles with well established LSPR and field-enhancement properties. The cultured skin cells were exposed to protoporphyrin IX and gold nanoparticles and subsequently illuminated with red light. We investigated the differences in cell viability by tuning different parameters, such as incubation time and light dose. In order to find optimal parameters for specific targeting of tumour cells, we compared normal human epidermal keratinocytes with a human squamous skin cancer cell line. The study indicates significantly enhanced cell death in the presence of nanoparticles and important differences in treatment efficiency between normal and tumour cells. These results are thus promising and clearly motivate further development of nanoparticle enhanced clinical PDT treatment.

Nyckelord: Cell culture, Keratinocytes, Nanoparticles, Photodynamic therapy, Protoporphyrin, Singlet oxygen, Surface plasmon resonance, Tumour cells



Denna post skapades 2011-06-08. Senast ändrad 2017-10-03.
CPL Pubid: 141439

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för teknisk fysik, Bionanofotonik (2007-2015)

Ämnesområden

Fysikalisk kemi

Chalmers infrastruktur