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Structural studies of bio-molecules and lipid assemblies using polarized light spectroscopy

Maxim Kogan (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Göteborg : Chalmers University of Technology, 2011. ISBN: 1652-943X.- 58 s.
[Licentiatavhandling]

The focus of the research presented in this thesis is structural studies of biological macromolecules using Linear Dichroism (LD) spectroscopy. The first part covers the flow alignment and structure of DMPC/DHPC phospholipid aggregates. By using membrane probes, we investigate the orientation behaviour of the lipid phase over a broad range of conditions such as temperature, hydration and shear gradient. The biophysical application of DMPC/DHPC membrane systems for structural studies of membrane bound proteins is demonstrated for the Cytochrome C protein. Second part of this thesis concerns the intercalation of a bidppz-bridged binuclear ruthenium complex (ΔΔ-P) into imperfectly paired DNA. We find that incorporation of an unpaired region in an oligonucleotide duplex strongly reduces the activation barrier, suggesting that such a structure may be proposed as a model for the DNA intercalation transition state.

Nyckelord: linear dichroism, spectroscopy, ruthenium complex, DNA breathing, lipid membrane, phospholipids



Denna post skapades 2011-05-18.
CPL Pubid: 140828

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Biofysikalisk kemi
Kinetik
Spektroskopi

Chalmers infrastruktur

Examination

Datum: 2011-06-09
Tid: 09:00
Lokal: KA

Ingår i serie

Thesis for the degree of licentiate of engineering - Department of Chemistry and Bioscience/Organic Chemistry, Chalmers University of Technology