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A spectroscopic study of 2-[4′-(Dimethylamino)phenyl]-benzothiazole Binding to Insulin Amyloid Fibrils

Catherine Kitts (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; D. A. Vanden Bout
Journal of Fluorescence (10530509). Vol. 20 (2010), 4, p. 881-889.
[Artikel, refereegranskad vetenskaplig]

The spectroscopic properties of 2-[4′-(dimethylamino)phenyl]- benzothiazole (BTA-2) in solution and in the presence of amyloid fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH3CN). BTA-2 binds to amyloid fibrils in solution leading to a characteristic blue-shift in the emission spectrum and an increase in fluorescence intensity. However, in solutions with increasing CH3CN concentration, there was a marked decrease in binding of the BTA-2 to fibrils. Studies demonstrating the effect of BTA-2 concentration on binding were performed. A comparison with the standard amyloid fluorescent marker, thioflavin T (ThT), showed that BTA-2 is more fluorescent, making it an excellent dye to label amyloid samples.

Nyckelord: 2-[4′-(Dimethylamino)phenyl]-benzothiazole, Amyloid fibrils; BTA-2, Fluorescence, Thioflavin T

Denna post skapades 2011-04-20.
CPL Pubid: 139526


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)


Fysikalisk kemi

Chalmers infrastruktur