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Ruthenium(II) Complex Enantiomers as Cellular Probes for Diastereomeric Interactions in Confocal and Fluorescence Lifetime Imaging Microscopy

Frida Svensson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Maria Abrahamsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; N. Stromberg ; A. G. Ewing ; Per Lincoln (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Journal of Physical Chemistry Letters (1948-7185). Vol. 2 (2011), 5, p. 397-401.
[Artikel, refereegranskad vetenskaplig]

Ruthenium dipyridophenazine (dppz) complexes are sensitive luminescent probes for hydrophobic environments. Here, we apply multi-frequency fluorescence lifetime imaging microscopy (FLLM) to Delta and Lambda enantiomers of lipophilic ruthenium dppz complexes in live and fixed cells, and their different lifetime staining patterns are related to conventional intensity-based microscopy. Excited-state lifetimes of the enantiomers determined from FLIM measurements correspond well with spectroscopically measured emission decay curves in pure microenvironments of DNA, phospholipid membranes, or a model protein. We show that FLIM can be applied to monitor the long-lived excited states of ruthenium complex enantiomers and, combined with confocal microscopy, give new insight into their biomolecular binding and reveal differences in the microenvironment probed by the complexes.

Nyckelord: dna-binding geometries, light-switch, dipyridophenazine complexes, polypyridyl complex, linear dichroism, membrane, picosecond, mechanism, solvents



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Denna post skapades 2011-04-08. Senast ändrad 2012-02-22.
CPL Pubid: 138836

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Nanovetenskap och nanoteknik
Fysikalisk kemi

Chalmers infrastruktur