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Coherent anti-Stokes Raman scattering microscopy of human smooth muscle cells in bioengineered tissue scaffolds

Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Maricris Esguerra ; Daniel Olausson ; Dick Delbro ; Alexandra Krettek ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Journal of Biomedical Optics (1560-2281). Vol. 16 (2011), 2, p. 021115.
[Artikel, refereegranskad vetenskaplig]

The integration of living, human smooth muscle cells in biosynthesized cellulose scaffolds was monitored by nonlinear microscopy toward contractile artificial blood vessels. Combined coherent anti-Stokes Raman scattering (CARS) and second harmonic generation (SHG) microscopy was applied for studies of the cell interaction with the biopolymer network. CARS microscopy probing CH(2)-groups at 2845 cm(-1) permitted three-dimensional imaging of the cells with high contrast for lipid-rich intracellular structures. SHG microscopy visualized the fibers of the cellulose scaffold, together with a small signal obtained from the cytoplasmic myosin of the muscle cells. From the overlay images we conclude a close interaction between cells and cellulose fibers. We followed the cell migration into the three-dimensional structure, illustrating that while the cells submerge into the scaffold they extrude filopodia on top of the surface. A comparison between compact and porous scaffolds reveals a migration depth of <10 μm for the former, whereas the porous type shows cells further submerged into the cellulose. Thus, the scaffold architecture determines the degree of cell integration. We conclude that the unique ability of nonlinear microscopy to visualize the three-dimensional composition of living, soft matter makes it an ideal instrument within tissue engineering.



Denna post skapades 2011-04-01. Senast ändrad 2017-01-27.
CPL Pubid: 138509

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)
Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning, Avdelningen för kirurgi (GU)
Institutionen för medicin, avdelningen för molekylär och klinisk medicin (GU)
Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)

Ämnesområden

MEDICIN OCH HÄLSOVETENSKAP

Chalmers infrastruktur