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The Influence of Microgravity on Invasive Growth in Saccharomyces cerevisiae

S. E. Van Mulders ; C. Stassen ; L. Daenen ; B. Devreese ; Verena Siewers (Institutionen för kemi- och bioteknik, Systembiologi) ; R. G. E. van Eijsden ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi) ; F. R. Delvaux ; R. Willaert
Astrobiology (1531-1074). Vol. 11 (2011), 1, p. 45-55.
[Artikel, refereegranskad vetenskaplig]

This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the Sigma 1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the Sigma 1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The Sigma 1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.

Nyckelord: Microgravity, Saccharomyces cerevisiae, Adhesion, Invasive growth, Budding, Proteomics, shear modeled microgravity, international-space-station, gene-expression, candida-albicans, escherichia-coli, biofilm formation, stress-response, immune-system, cell-cell, yeast



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Denna post skapades 2011-03-21. Senast ändrad 2014-10-27.
CPL Pubid: 138221

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Livsvetenskaper
Biologiska vetenskaper

Chalmers infrastruktur