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Chemical imaging of glucose by CARS microscopy

Madeleine Åkeson (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Lena Gustafsson (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Journal of Raman Spectroscopy (0377-0486). Vol. 41 (2010), 12, p. 1348-1354.
[Artikel, refereegranskad vetenskaplig]

Glucose is one of the most fundamental molecules within life and bioengineering sciences. Present understanding of its role in cellular and bioengineering processes relies primarily on invasive, large-scale biochemical analysis, providing no spatial information on glucose pools or fluxes. This work identifies an emerging microscopy technique based on coherent anti-Stokes Raman scattering (CARS), which fulfills the need of quantitative imaging of glucose at the single-cell level with submicrometer resolution. No sample preparation with reporter molecules is required, ensuring that the low-weight metabolite is studied under natural conditions. The potential of CARS microscopy is illustrated by quantitatively mapping glucose fluxes and distributions in a microfluidic bioreactor and in lipid-bilayer vesicles, the latter as a model for glucose transmembrane transport. Furthermore, the metabolic response to a glucose pulse was monitored in living yeast cells. This study signifies a new era within CARS microscopy for its use of monitoring carbohydrates, in particular glucose which is one of the most abundant molecules in nature.

Nyckelord: coherent anti-Stokes Raman scattering (CARS), CARS microscopy, carbohydrates, glucose, liposome, yeast, raman scattering microscopy, saccharomyces-cerevisiae, insulin-resistance, spectroscopy, cells, stress, yeast, microspectroscopy, visualization, transporters



Denna post skapades 2011-03-04. Senast ändrad 2016-08-16.
CPL Pubid: 137591

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)

Ämnesområden

Kemi

Chalmers infrastruktur