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Visualization of β-carotene and starch granules in plant cells using CARS and SHG microscopy

Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Anton Bengtsson (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Marie Alminger (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Ulf Svanberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Journal of Raman Spectroscopy (0377-0486). Vol. 42 (2010), 4, p. 586-592.
[Artikel, refereegranskad vetenskaplig]

Information on the content and bioavailability of provitamin A carotenoids, such as beta-carotene, in plant foods is of great interest due to the widespread vitamin A deficiency in developing countries. While the amount of beta-carotene can readily be quantified with analytical techniques, there is limited information on beta-carotene morphology in native plant materials. Here, we introduce nonlinear microscopy for three-dimensional, label-free imaging of carotenoids in fresh and thermally treated plant tissues, providing quantitative information at single-aggregate level and detailed insight into their distribution. Carotenoids in orange-fleshed sweet potato (OFSP), carrot, and mango were visualized by coherent anti-Stokes Raman scattering (CARS) microscopy and, in the case of OFSP, related to the plant-matrix morphology by simultaneous second-harmonic generation (SHG) microscopy of starch granules. Sizes, shapes, densities, and location of different types of carotenoid bodies were quantified. While OFSP and carrot showed heterogeneous rod-shaped bodies with high carotenoid densities indicated by higher CARS signals, the carotenoid-filled lipid droplets in mango appeared as homogeneous low-density aggregates of rounded shape. In addition, beta-carotene densities and morphologies in OFSP were studied after thermal processing, showing that the bodies remain intact despite significant changes of the surrounding starch granules.

Nyckelord: coherent anti-Stokes Raman scattering; second-harmonic generation; microscopy; carotenoids; plant cells

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Denna post skapades 2011-02-01. Senast ändrad 2017-01-27.
CPL Pubid: 136353


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)
Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)



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