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Fungal Ferulic Acid Esterases – Specificity and Phylogeny

Hampus Sunner (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Lisbeth Olsson (Institutionen för kemi- och bioteknik, Industriell Bioteknik ) ; Gianni Panagiotou (Institutionen för kemi- och bioteknik, Industriell Bioteknik )
Italic5 Science and Technology of Biomasses Proceedings Book, M Orlandi, C Crestine (Ed.). Italic5/COST conference, Sept 1-4 2009, Varenna, Italy (2009)
[Konferensbidrag, poster]

Ferulic Acid Esterases (FAE) is a large heterogeneous group of enzymes with activity on esters of hydroxy- and metoxy- substituted cinnamic acid derivatives, such as ferulic acid. These ester bonds occur in the cell walls of plants and are especially common in grasses. As little systematic knowledge has been collected about this group of enzymes and only a few enzymes have been biochemically characterised to date, we have explored the phylogeny of FAEs using bioinformatic tools. We can conclude that the known Ferulic Acid Esterases belong to several evolutionary distant groups, two of which have dozens of highly related sequences, and a few groups with no members other than the known enzyme. The phylogeny also suggests certain similarities of substrate specificity within groups and proposes enzymes, whose biochemical characterisation would be especially informative for our understanding of the FAE families.

Nyckelord: Ferulic Acid Esterase, Phylogeny, Enzyme characterisation, BLAST, PhyML



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Denna post skapades 2011-01-10. Senast ändrad 2016-05-24.
CPL Pubid: 132853

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Industriell Bioteknik (2008-2014)

Ämnesområden

Energi
Livsvetenskaper
Bioinformatik och systembiologi
Industriell bioteknik
Biokatalys och enzymteknik
Strukturbiokemi

Chalmers infrastruktur