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Nonlinear microscopy of lipid storage and fibrosis in muscle and liver tissues of mice fed high-fat diets

Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Britt G. Gabrielsson (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Fredrik Svedberg (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Agneta Holmäng ; Ann-Sofie Sandberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär mikroskopi)
Journal of Biomedical Optics (1083-3668). Vol. 15 (2010), 6, p. 066008-1 - 066008-10.
[Artikel, refereegranskad vetenskaplig]

Hallmarks of high-fat Western diet intake, such as excessive lipid accumulation in skeletal muscle and liver as well as liver fibrosis, are investigated in tissues from mice using nonlinear microscopy, second harmonic generation (SHG), and coherent anti-Stokes Raman scattering (CARS), supported by conventional analysis methods. Two aspects are presented; intake of standard chow versus Western diet, and a comparison between two high-fat Western diets of different polyunsaturated lipid content. CARS microscopy images of intramyocellular lipid droplets in muscle tissue show an increased amount for Western diet compared to standard diet samples. Even stronger diet impact is found for liver samples, where combined CARS and SHG microscopy visualize clear differences in lipid content and collagen fiber development, the latter indicating nonalcoholic fatty liver disease (NAFLD) and steatohepatitis induced at a relatively early stage for Western diet. Characteristic for NAFLD, the fibrous tissue-containing lipids accumulate in larger structures. This is also observed in CARS images of liver samples from two Western-type diets of different polyunsaturated lipid contents. In summary, nonlinear microscopy has strong potential (further promoted by technical advances toward clinical use) for detection and characterization of steatohepatitis already in its early stages.

Nyckelord: cellular biophysics, coherent antiStokes Raman scattering, diseases, harmonic generation, lipid bilayers, liver, muscle, optical microscopy



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Denna post skapades 2010-12-03. Senast ändrad 2015-02-06.
CPL Pubid: 130030

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)
Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)
Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi (GU)

Ämnesområden

Livsvetenskaper
Medicinsk teknik
Molekylär medicin (genetik och patologi)
Endokrinologi

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