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Properties and Applications of Shear-Driven Lipid Bilayers

Peter Jönsson (Institutionen för teknisk fysik, Biologisk fysik)
Göteborg : Chalmers University of Technology, 2010. ISBN: 978-91-7385-462-7.- 180 s.
[Doktorsavhandling]

A supported lipid bilayer (SLB) is a lipid bilayer formed on a solid substrate. This system is currently one of the most common model systems used to imitate the properties of the cell membrane that surrounds human cells. In this work it was found that the hydrodynamic shear force from a liquid flow above an SLB can be used to move the entire SLB in the direction of the flowing liquid. This takes place in a rolling type of motion, with the lower monolayer of the SLB being essentially stationary on the support. Forming the SLB on the walls of a microfluidic channel provides a controlled and versatile method of handling and transporting the SLB and molecules associated with it. The theory behind, and some general observations, of this phenomenon are presented in this thesis. In addition of being able to drive the SLB over different types of surfaces and structures, this technique can also be used to manipulate the concentration of molecules solubilized in, or coupled to, the SLB. For example, it was found that membrane-bound proteins could be accumulated at the front of the advancing SLB, leading to a local increase in surface concentration of several orders of magnitude. This could allow the detection of membrane-associated molecules at much lower average surface coverage than previously possible. Another observation was that different types of membrane-associated molecules have a characteristic drift velocity in the lipid bilayer, under the same bulk flow in the channel. This opens up the possibility of separating membrane-associated molecules within the SLB based on differences in drift velocity, as in chromatography, but without the need of first extracting the molecules of interest from the lipid bilayer.

Nyckelord: lipid bilayer, cell membrane, microfluidics, fluorescence microscopy, photobleaching, diffusivity, mobility, model systems, simulations, membrane proteins



Denna post skapades 2010-11-03. Senast ändrad 2014-09-10.
CPL Pubid: 128572

 

Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Biofysik

Chalmers infrastruktur

Relaterade publikationer

Inkluderade delarbeten:


Mechanical Behavior of a Supported Lipid Bilayer under External Shear Forces


Shear-Driven Motion of Supported Lipid Bilayers in Microfluidic Channels


Sealing of Submicrometer Wells by a Shear-Driven Lipid Bilayer


Accumulation and Separation of Membrane-Bound Proteins Using Hydrodynamic Forces


Effects of Surface Pressure and Internal Friction on the Dynamics of Shear-Driven Supported Lipid Bilayers


Examination

Datum: 2010-12-09
Tid: 13:15
Lokal: FB-salen, Fysikgården 4, Chalmers tekniska högskola.
Opponent: Prof. Joachim Rädler, Department of Physics, Ludwig-Maximilians-University, Germany.

Ingår i serie

Doktorsavhandlingar vid Chalmers tekniska högskola. Ny serie 3143