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Single-cell electroporation

M. Y. Wang ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Jessica Olofsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; S. G. Weber
Analytical and Bioanalytical Chemistry (1618-2642). Vol. 397 (2010), 8, p. 3235-3248.
[Artikel, refereegranskad vetenskaplig]

Single-cell electroporation (SCEP) is a relatively new technique that has emerged in the last decade or so for single-cell studies. When a large enough electric field is applied to a single cell, transient nano-pores form in the cell membrane allowing molecules to be transported into and out of the cell. Unlike bulk electroporation, in which a homogenous electric field is applied to a suspension of cells, in SCEP an electric field is created locally near a single cell. Today, single-cell-level studies are at the frontier of biochemical research, and SCEP is a promising tool in such studies. In this review, we discuss pore formation based on theoretical and experimental approaches. Current SCEP techniques using microelectrodes, micropipettes, electrolyte-filled capillaries, and microfabricated devices are all thoroughly discussed for adherent and suspended cells. SCEP has been applied in in-vivo and in-vitro studies for delivery of cell-impermeant molecules such as drugs, DNA, and siRNA, and for morphological observations.

Nyckelord: Cell membrane, Electric field, Microelectrodes, Microfluidics, Transfection, neurons in-vivo, mammalian-cells, electric-field, micro-electroporation, patch-clamp, membrane electropermeabilization, modeling electroporation, genetic manipulation, ellipsoidal cells, filled capillary

Denna post skapades 2010-08-26.
CPL Pubid: 125227


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



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