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Quartz crystal microbalance with dissipation monitoring of supported lipid bilayers on various substrates

N. J. Cho ; C. W. Frank ; Bengt Kasemo (Institutionen för teknisk fysik, Kemisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Nature Protocols (1754-2189). Vol. 5 (2010), 6, p. 1096-1106.
[Artikel, refereegranskad vetenskaplig]

Supported lipid bilayers (SLBs) mimic biological membranes and are a versatile platform for a wide range of biophysical research fields including lipid-protein interactions, protein-protein interactions and membrane-based biosensors. the quartz crystal microbalance with dissipation monitoring (QCM-D) has had a pivotal role in understanding SLB formation on various substrates. as shown by its real-time kinetic monitoring of SLB formation, QCM-D can probe the dynamics of biomacromolecular interactions. We present a protocol for constructing zwitterionic SLBs supported on silicon oxide and titanium oxide, and discuss technical issues that need to be considered when working with charged lipid compositions. Furthermore, we explain a recently developed strategy that uses an amphipathic, a-helical (AH) peptide to form SLBs on gold and titanium oxide substrates. the protocols can be completed in less than 3 h.

Nyckelord: surface-plasmon resonance, c-virus ns5a, vesicle adsorption, phospholipid-bilayers, qcm-d, in-situ, biomolecular adsorption, phosphatidyl serine, protein adsorption, size distribution



Denna post skapades 2010-06-18. Senast ändrad 2014-03-24.
CPL Pubid: 122968

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Kemisk fysik (1900-2015)
Institutionen för teknisk fysik, Biologisk fysik (2007-2015)

Ämnesområden

Fysik

Chalmers infrastruktur