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A Microfluidic Pipette for Single-Cell Pharmacology

Alar Ainla (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Erik T. Jansson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Natalia Stepanyants (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Aldo Jesorka (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Analytical Chemistry (0003-2700). Vol. 82 (2010), 11, p. 4529-4536.
[Artikel, refereegranskad vetenskaplig]

We report on a free-standing microfluidic pipette made in poly(dimethylsiloxane) having a circulating liquid tip that generates a self-con-fining volume in front of the outlet channels. The method is flexible and scalable as the geometry and the size of the recirculation zone is defined by pressure, channel number, and geometry. The pipette is capable of carrying out a variety of complex fluid processing operations, such as mixing, multiplexing, or gradient generation at selected cells in cell and tissue cultures. Using an uptake assay, we show that it is possible to generate dose response curves in situ from adherent Chinese hamster ovary cells expressing proton-activated human transient receptor potential vanilloid (hTRPV1) receptors. Using confined superfusion and cell stimulation, we could activate hTRPV1 receptors in single cells, measure the response by a patch-clamp pipette, and induce membrane bleb formation by exposing selected groups of cells to formaldehyde/dithiothreitol-containing solutions, respectively. In short, the microfluidic pipette allows for complex, contamination-free multiple-compound delivery for pharmacological screening of intact adherent cells.

Nyckelord: plasma-membrane, capsaicin receptor, ion-channel, stimulation, resolution, currents

Denna post skapades 2010-06-18. Senast ändrad 2016-04-28.
CPL Pubid: 122945


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Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



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