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Intravital fluorescent microscopic evaluation of bacterial cellulose as scaffold for vascular grafts.

Maricris Esguerra ; Helen Fink ; Matthias W Laschke ; Anders Jeppsson ; Dick Delbro ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Michael D Menger ; Bo Risberg
Journal of biomedical materials research. Part A (1552-4965). Vol. 93 (2010), 1, p. 140-9.
[Artikel, refereegranskad vetenskaplig]

Although commonly used synthetic vascular grafts perform satisfactorily in large caliber blood vessels, they are prone to thrombosis in small diameter vessels. Therefore, small vessels might benefit from tissue engineered vascular grafts. This study evaluated bacterial cellulose (BC) as a potential biomaterial for biosynthetic blood vessels. We implanted the dorsal skinfold chambers in three groups of Syrian golden hamsters with BC (experimental group), polyglycolic acid, or expanded polytetrafluorethylene (control groups). Following implantation, we used intravital fluorescence microscopy, histology, and immunohistochemistry to analyze the biocompatibility, neovascularization, and incorporation of each material over a time period of 2 weeks. Biocompatibility was good in all groups, as indicated by the absence of leukocyte activation upon implantation. All groups displayed angiogenic response in the host tissue, but that response was highest in the polyglycolic acid group. Histology revealed vascularized granulation tissue surrounding all three biomaterials, with many proliferating cells and a lack of apoptotic cell death 2 weeks after implantation. In conclusion, BC offers good biocompatibility and material incorporation compared with commonly used materials in vascular surgery. Thus, BC represents a promising new biomaterial for tissue engineering of vascular grafts.

Nyckelord: Acetobacter, chemistry, Animals, Blood Vessel Prosthesis, Cell Adhesion, drug effects, Cellulose, pharmacology, Cricetinae, Female, Hemodynamics, drug effects, Immunohistochemistry, Inflammation, pathology, Leukocyte Rolling, drug effects, Materials Testing, Mesocricetus, Microscopy, Fluorescence, methods, Microvessels, drug effects, physiology, Neovascularization, Physiologic, drug effects, Tissue Scaffolds, chemistry



Denna post skapades 2010-05-28. Senast ändrad 2012-03-01.
CPL Pubid: 122024

 

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Institutioner (Chalmers)

Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning, Avdelningen för kirurgi (GU)
Institutionen för medicin, avdelningen för akut och kardiovaskulär medicin (2006-2011)
Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)

Ämnesområden

MEDICIN OCH HÄLSOVETENSKAP
Kirurgi

Chalmers infrastruktur