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Nanotube-vesicle networks with functionalized membranes and interiors

M. Davidson ; M. Karlsson ; J. Sinclair ; Kristin Sott ; Owe Orwar (Institutionen för fysikalisk kemi)
J Am Chem Soc (0002-7863 (Print) 0002-7863 (Linking)). Vol. 125 (2003), 2, p. 374-8.
[Artikel, refereegranskad vetenskaplig]

We describe nanotube-vesicle networks with reconstituted membrane protein from cells and with interior activity defined by an injection of microparticles or molecular probes. The functionality of a membrane protein after reconstitution was verified by single-channel ion conductance measurements in excised inside-out patches from the vesicle membranes. The distribution of protein, determined by fluorescence detection, in the network membrane was homogeneous and could diffuse via a nanotube connecting two vesicles. We also show how injecting small unilamellar protein-containing vesicles can differentiate the contents of individual containers in a network. The combination of membrane activity and interior activity was demonstrated by ionophore-assisted accumulation, and internal Calcium Green-mediated detection, of Ca2+ within a single network container. This system can model a variety of biological functions and complex biological multicompartment structures and might serve as a platform for constructing complex sensor and computational devices.

Nyckelord: Biomimetic Materials/*chemistry, Biosensing Techniques, Erythrocyte Membrane/chemistry/metabolism, Humans, Liposomes/*chemistry, Membrane Proteins/*chemistry/metabolism, Microscopy, Fluorescence, Nanotechnology/*methods, Organelles/chemistry/metabolism, Signal Transduction



Denna post skapades 2010-03-01. Senast ändrad 2010-04-30.
CPL Pubid: 116806

 

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Institutioner (Chalmers)

Institutionen för fysik (GU) (GU)
Institutionen för fysikalisk kemi (1900-2003)

Ämnesområden

Kemi

Chalmers infrastruktur