CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae

D. B. Flagfeldt ; V. Siewers ; L. Huang ; Jens B. Nielsen (Institutionen för kemi- och bioteknik, Systembiologi)
Yeast (0749-503X). Vol. 26 (2009), 10, p. 545-551.
[Artikel, refereegranskad vetenskaplig]

The construction of mitotically stable yeast strains for heterologous gene or pathway expression often requires chromosomal integration. However, transcription levels vary between different chromosome regions. We therefore characterized 20 different integration sites of the Sacchromyces cerevisiae genome by inserting lacZ as a reporter gene under the control of two different promoters and determining expression levels through enzyme activity measurement. An up to 8.7-fold difference was detected between the sites conferring lowest and highest expression, respectively. This opens the opportunity for modulating gene expression levels while retaining promoter and culture conditions. Copyright (C) 2009 John Wiley & Sons, Ltd.

Nyckelord: reporter gene, lacZ, solo LTRs, Saccharomyces cerevisiae, escherichia-coli, structural gene, beta-carotene, yeast, genome, decarboxylase, specificity, precursor, sequence, pathway

Den här publikationen ingår i följande styrkeområden:

Läs mer om Chalmers styrkeområden  

Denna post skapades 2010-02-24. Senast ändrad 2014-10-27.
CPL Pubid: 114533


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)


Industriell bioteknik

Chalmers infrastruktur