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Liposome-Based Chemical Barcodes for Single Molecule DNA Detection Using Imaging Mass Spectrometry

Anders Gunnarsson (Institutionen för teknisk fysik, Biologisk fysik) ; Peter Sjövall (Institutionen för teknisk fysik, Biologisk fysik) ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Nano Letters (1530-6984). Vol. 10 (2010), 2, p. 732-737.
[Artikel, refereegranskad vetenskaplig]

We report on a mass-spectrometry (time-of-flight secondary ion mass spectrometry, TOF-SIMS) based method For multiplexed DNA detection utilizing a random array, where the lipid composition of small unilamellar liposomes act as chemical barcodes to identify unique DNA target sequences down to the single molecule level. In a sandwich format, suspended target-DNA to be detected mediates the binding of capture-DNA modified liposomes to surface-immobilized probe-DNA. With the lipid composition of each liposome encoding a unique target-DNA sequence, TOF-SIMS analysis was used to determine the chemical fingerprint of the bound liposomes. Using high-resolution TOF-SIMS imaging, providing sub-200 nm spatial resolution, single DNA targets could be detected and identified via the chemical fingerprint of individual liposomes. The results also demonstrate the capability of TOF-SIMS to provide multiplexed detection of DNA targets on substrate areas in the micrometer range. Together with a high multiplexing capacity, this makes the concept an interesting alternative to existing barcode concepts based on fluorescence, Raman, or graphical codes for small-scale bioanalysis.

Nyckelord: TOF-SIMS, liposome, DNA, barcode, single molecule detection, high-density, nanoparticle probes, maldi-tof, hybridization, expression, targets, arrays, sims

Denna post skapades 2010-02-23.
CPL Pubid: 114080


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Institutionen för teknisk fysik, Biologisk fysik (2007-2015)



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