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Steady-State Electrochemical Determination of Lipidic Nanotube Diameter Utilizing an Artificial Cell Model

Kelly L. Adams ; Johan Engelbrektsson ; Marina V. Voinova ; Bo Zhang ; Daniel J. Eves ; Roger Karlsson ; Michael L. Heien ; Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Andrew G Ewing
Analytical Chemistry (1520-6882). Vol. 82 (2010), 3, p. 1020-1026.
[Artikel, refereegranskad vetenskaplig]

By exploiting the capabilities of steady-state electrochemical measurements, we have measured the inner diameter of a lipid nanotube using Fick’s first law of diffusion in conjunction with an imposed linear concentration gradient of electroactive molecules over the length of the nanotube. Fick’s law has been used in this way to provide a direct relationship between the nanotube diameter and the measurable experimental parameters Δi (change in current) and nanotube length. Catechol was used to determine the Δi attributed to its flux out of the nanotube. Comparing the nanotube diameter as a function of nanotube length revealed that membrane elastic energy was playing an important role in determining the size of the nanotube and was different when the tube was connected to either end of two vesicles or to a vesicle on one end and a pipet tip on the other. We assume that repulsive interaction between neck regions can be used to explain the trends observed. This theoretical approach based on elastic energy considerations provides a qualitative description consistent with experimental data.



Denna post skapades 2010-01-29. Senast ändrad 2015-02-26.
CPL Pubid: 111200

 

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Institutioner (Chalmers)

Institutionen för kemi (2001-2011)
Institutionen för fysik (GU) (GU)
Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Biologisk fysik
Analytisk kemi
Biofysikalisk kemi

Chalmers infrastruktur