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Parallel dual-color fluorescence cross-correlation spectroscopy using diffractive optical elements

M. Gösch ; H. Blom ; S. Anderegg ; K. Korn ; P. Thyberg ; M. Wells ; T. Lasser ; R. Rigler ; A. Magnusson ; Sverker Hård (Institutionen för mikroteknologi och nanovetenskap, Fotonik)
Journal of Biomedical Optics (10833668). Vol. 10 (2005), 5,
[Artikel, refereegranskad vetenskaplig]

Dual-color cross-correlation spectroscopy allows the detection and quantification of labeled biomolecules at ultra-low concentrations, whereby the sensitivity of the assay correlates with the measurement time. We now describe a parallel multifocal dual-color spectroscopic configuration employing multiple avalanche photodiodes and hardware correlators. Cross-correlation curves are obtained from several dual-color excitation foci simultaneously. Multifocal dual-color excitation is achieved by splitting each of two laser beams (488 and 633 nm) into four sub-beams with the help of two 2 × 2 fan-out diffractive optical elements (DOEs), and subsequent superposition of the two sets of four foci. The fluorescence emission from double-labeled biomolecules is detected by two 2 × 2 fiber arrays.



Denna post skapades 2010-01-27.
CPL Pubid: 111035

 

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Institutioner (Chalmers)

Institutionen för mikroteknologi och nanovetenskap, Fotonik

Ämnesområden

Elektroteknik och elektronik

Chalmers infrastruktur