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Expression of delta opioid receptor mRNA and protein in the rat cerebral cortex and cerebellum is decreased by growth hormone.

Anders I. Persson ; N David Åberg ; Jan Oscarsson ; Olle Isaksson ; Lars Rönnbäck ; Fredrik Frick ; Christian Sonesson (Institutionen för matematisk statistik) ; Peter S Eriksson
Journal of neuroscience research (0360-4012). Vol. 71 (2003), 4, p. 496-503.
[Artikel, refereegranskad vetenskaplig]

Hormones released from the pituitary have been shown to regulate the expression of different proteins in the central nervous system. We wanted to examine whether peripheral administration of bovine growth hormone (bGH) regulates the expression of delta-opioid receptor (DOR) in the cerebral cortex and cerebellum. Expression of the DOR protein was quantified using Western blot densitometry. DOR mRNA was quantified with a solution hybridization RNase protection assay. Hypophysectomized (Hx) and untreated normal female rats were included in the study. All Hx rats were hormonally treated with cortisol (400 microg/kg/day) and L-thyroxine (10 microg/kg/day) for 19 days. Hypophysectomy resulted in a threefold increase in cerebral cortex and a twofold increase in cerebellum of the DOR protein compared with normal rats. One subgroup of Hx rats received bGH (1 mg/kg body weight) as a daily subcutaneous injection for 19 days. This treatment normalized the levels of DOR protein in the cerebral cortex and cerebellum. Immunohistochemical experiments showed that GH decreased DOR expression especially in layers II-VI in cerebral cortex and in stratum moleculare in cerebellum. Quantification of DOR mRNA by solution hybridization RNase protection assay corresponded to the DOR protein measurements. We conclude that the expression of DORs in cerebral cortex and cerebellum is regulated by GH.

Nyckelord: Animals, Cattle, Cerebellum, chemistry, drug effects, metabolism, Cerebral Cortex, chemistry, drug effects, metabolism, Female, Gene Expression Regulation, drug effects, physiology, Growth Hormone, pharmacology, Protein Biosynthesis, Proteins, analysis, RNA, Messenger, analysis, biosynthesis, Rats, Rats, Sprague-Dawley, Receptors, Opioid, delta, analysis, biosynthesis

Denna post skapades 2010-01-22. Senast ändrad 2013-02-07.
CPL Pubid: 110216


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Institutioner (Chalmers)

Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap (1999-2005)
Institutionen för fysiologi och farmakologi, Avdelningen för fysiologi (1991-2005)
Institutionen för invärtesmedicin, Avdelningen för internmedicin (1991-2005)
Institutionen för matematisk statistik (2002-2004)
Institutionen för klinisk neurovetenskap (1991-2005)



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