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Gas-chromatographic analysis of sugars and related hydroxy acids as acyclic oxime and ester trimethylsilyl derivatives

Göran Petersson (Institutionen för teknisk kemi)
Carbohydrate Research Vol. 33 (1974), p. 47-61.
[Artikel, refereegranskad vetenskaplig]

A g.l.c. method which makes possible the simultaneous analysis of the common types of acidic and neutral sugars is described. Particular advantages are achieved with hydroxy acids, ketoses, and uronic acids. Acids and lactones are first converted into their sodium salts. Free or potential aldehydo and keto groups are transformed into oximes or O-methyloximes. Fully substituted acyclic ester and oxime trimethylsilyl derivatives are prepared with bis(trimethylsilyl)trifluoroacetamide and chlorotrimethylsilane. Syn and anti isomers of the oximes are formed, but a stationary phase can often be chosen on which the two peaks coincide. The silicone stationary phases OV-1, OV-17, QF-1, and XE-60 were used. Retention data are given for aldonic and deoxyaldonic acids, aldoses, ketoses, and uronic acids. Relationships between structure and retention are discussed. The derivatives are suited to structural analysis by gas chromatography—mass spectrometry.

Nyckelord: monosaccharides, oximes, hydroxy acids, Me3Si derivatives, gas chromatography, silicone phases, mass spectra


The described uniform chromatographic approach facilitates both gas chromatographic separation and mass spectrometric identification of the many important monosaccharides and hydroxy acids. The article was central in the doctoral thesis of Göran Petersson from 1974 because it applies to most of the ompounds studied in the thesis.



Denna post skapades 2010-01-21. Senast ändrad 2011-06-20.
CPL Pubid: 109871

 

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Institutionen för teknisk kemi (1946-1998)

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Gas chromatography - mass spectrometry of sugars and related hydroxy acids as trimethylsilyl derivatives