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Accumulation of FITC near stratum corneum-visualizing epidermal distribution of a strong sensitizer using two-photon microscopy

Kristin Samuelsson ; Carl Simonsson ; Charlotte A Jonsson ; Gunnar Westman (Institutionen för kemi- och bioteknik, Organisk kemi) ; Marica B Ericson ; Ann-Therese Karlberg
Contact Dermatitis (0105-1873). Vol. 61 (2009), 2, p. 91-100.
[Artikel, refereegranskad vetenskaplig]

Background The allergenic potency of a hapten is related to its skin penetration properties, but little is known about the distribution of haptens in the skin following topical application. Objectives The aim of this study was to investigate the diffusion and epidermal distribution using two-photon microscopy (TPM) of two fluorescent compounds. Methods Sensitizing capacities of fluorescein isothiocyanate (FITC) and fluorescein were investigated using the local lymph node assay. Chemical reactivity of the compounds was analysed, and their distribution in human epidermis was visualized using TPM and confocal microscopy. Also the in vitro diffusion through epidermis of FITC and fluorescein has been examined. Results FITC was classified as an extreme sensitizer, whereas fluorescein was non-sensitizing. TPM and confocal microscopy showed an accumulation of FITC in stratum corneum (SC), whereas fluorescein was more evenly distributed in epidermis. The diffusion of fluorescein through epidermis was three times higher than that of FITC. Conclusions TPM, which has never been used in this context before, is a promising tool for visualizing the distribution of fluorescent compounds of varying reactivity in intact skin. The strong allergen FITC is mainly retained in or adjacent to SC, whereas most fluorescein diffused through the epidermis.

Nyckelord: contact allergy, FITC, fluorescent hapten, laser scanning confocal, microscopy, local lymph node assay, skin penetration, two-photon, microscopy, lymph-node assay, allergic contact-dermatitis, in-vitro, skin, sensitization, peptide reactivity, fluorescein isothiocyanate, classification, dinitrochlorobenzene, phenylisothiocyanate, hypersensitivity

Denna post skapades 2010-01-14. Senast ändrad 2016-02-01.
CPL Pubid: 107226


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Institutioner (Chalmers)

Institutionen för kemi (2001-2011)
Institutionen för kemi- och bioteknik, Organisk kemi (2006-2014)
Institutionen för fysik (GU) (GU)
Institutionen för kliniska vetenskaper, sektionen för hud, plastik och öron (2006-2009)


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