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Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes

Michal Tokarz (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Björn Åkerman (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Jessica Olofsson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; J. F. Joanny ; P. Dommersnes ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Proceedings of the National Academy of Sciences of the United States of America (00278424). Vol. 102 (2005), 26, p. 9127-9132.
[Artikel, refereegranskad vetenskaplig]

We demonstrate a complete nanotube electrophoresis system (nanotube radii in the range of 50 to 150 nm) based on lipid membranes, comprising DNA injection, single-molecule transport, and single-molecule detection. Using gel-capped electrodes, electrophoretic single-file transport of fluorescently labeled dsDNA molecules is observed inside nanotubes. The strong confinement to a channel of molecular dimensions ensures a detection efficiency close to unity and identification of DNA size from its linear relation to the integrated peak intensity. In addition to constituting a nanotechnological device for identification and quantification of single macromolecules or biopolymers, this system provides a method to study their conformational dynamics, reaction kinetics, and transport in cell-like environments.

Denna post skapades 2010-01-12. Senast ändrad 2015-02-11.
CPL Pubid: 106595


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



Chalmers infrastruktur