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Blocking peptides decrease tissue transglutaminase processing of gliadin in vitro

Karolina Hoffmann (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Marie Alminger (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Thomas Andlid (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap) ; Tingsu Chen ; Olof Olsson ; Ann-Sofie Sandberg (Institutionen för kemi- och bioteknik, Livsmedelsvetenskap)
Journal of Agricultural and Food Chemistry (0021-8561). Vol. 57 (2009), 21, p. 10150-10155.
[Artikel, refereegranskad vetenskaplig]

Tissue transglutaminase (tTG) plays an important role in celiac disease pathology as it catalyzes deamidation and cross-linking of specific gluten peptides and converts them into potent epitopes recognized by intestinal T-cells. We investigated whether synthetic peptides with high affinity to gliadin could alter tTG activity on gliadin and whole gluten digest. The immobilized substrates were incubated with synthetic peptides identified by the phage display technique and a control peptide with no affinity to gliadin. Transglutaminase activity was measured with time resolved fluorescence. The mean tTG activity, compared to that of the control without the peptides, was reduced by 31, 33, and 36% for three selected gliadin-binding peptides, and 30% for the peptide pool (P <= 0.001-0.004) when gliadin was the substrate. Finally, substrate specificity experiments suggested that avenin was processed in a manner similar that used for gliadin during in vitro assays with tTG. The results showed that the blocking peptides efficiently reduced tTG processing of gliadin in vitro, and this strategy will be further investigated as an alternative therapy for celiac disease.

Nyckelord: celiac disease, gliadin, synthetic peptides, time resolved fluoroscence, tissue transglutaminase



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Denna post skapades 2009-11-09. Senast ändrad 2015-09-04.
CPL Pubid: 101425

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Livsmedelsvetenskap (2005-2014)
Institutionen för växt- och miljövetenskaper (2006-2011)

Ämnesområden

Livsvetenskaper
Biologiska vetenskaper
Immunologi inom det medicinska området

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