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Label-Free Measurements of Molecular Transport across Liposome Membranes using Evanescent-Wave Sensing

Magnus Brändén (Institutionen för teknisk fysik, Biologisk fysik) ; S. Dahlin ; Fredrik Höök (Institutionen för teknisk fysik, Biologisk fysik)
Chemphyschem (1439-4235). Vol. 9 (2008), 17, p. 2480-2485.
[Artikel, refereegranskad vetenskaplig]

Pucker up! A novel approach enabling direct measurements of biomolecular transfer across lipid bilayer membranes, using surface plasmon resonance, is demonstrated. The figure shows the transfer of sucrose (S) through melittin pores formed in surface-attached liposomes. By measuring the shift in refractive index in the volume enclosed by the liposomes, the sucrose transfer can be time-resolved and quantified.

Nyckelord: liposomes, melittin, membranes, surface plasmon resonance, transport, PLASMON RESONANCE SENSORS, DRUG DISCOVERY, LIPID VESICLES, MELITTIN, CHOLESTEROL, SPR, DNA

Denna post skapades 2009-10-26. Senast ändrad 2016-08-16.
CPL Pubid: 100768


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Institutioner (Chalmers)

Institutionen för teknisk fysik, Biologisk fysik (2007-2015)


Teknisk fysik

Chalmers infrastruktur