Natalia Mossberg (Institutionen för neurovetenskap och fysiologi, GU); Oluf Andersen (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi, GU); Staffan Nilsson (Institutionen för matematiska vetenskaper, matematisk statistik, Chalmers/GU); Claes Dahlgren (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning, GU); Kristoffer Hellstrand (Institutionen för biomedicin, avdelningen för infektionssjukdomar, GU); Magnus Lindh (Institutionen för biomedicin, avdelningen för infektionssjukdomar, GU); åke Svedhem (Institutionen för biomedicin, avdelningen för infektionssjukdomar, GU); Tomas Bergström (Institutionen för biomedicin, avdelningen för infektionssjukdomar, GU); Charlotta Movitz (Institutionen för biomedicin, avdelningen för infektionssjukdomar, GU)

Journal of Neuroimmunology, 192 ( 1-2 ) s. 186-191

0165-5728

Artikel, refereegranskad vetenskaplig

2007

engelska

http://dx.doi.org/10.1016/j.jneuroim.2007.09.020

The NADPH oxidase-dependent formation of reactive oxygen species ("oxygen radicals") by phagocytic cells constitutes an important part of the innate immune defence against microorganisms. Recent studies in animal models imply that a deficient function of the NADPH oxidase may be linked to the development of autoimmunity, but a link between oxygen radical production and severity of autoimmune disease in humans has not been established. We have examined the oxygen radical production in peripheral blood leukocytes from patients with the Guillain-Barre syndrome (GBS). Leukocytes from GBS patients in a stationary phase 1-5 years after their acute episode were activated by the formyl peptide receptor (FPR) ligand formyl-Met-Leu-Phe (fMLF) or the closely related formyl peptide like receptor 1 (FPRL1) ligand Trp-Lys-Tyr-Met-Val-Met-NH2 (WKYMVM). The patients were dichotomized according to severity by 1) the requirement of intensive care unit treatment and 2) the ability to walk independently after 3 months. Our data show that the amount of superoxide release following challenge with either of the two agonists fMLF and WKYMVM was significantly lower in patients requiring intensive care unit treatment or unable to walk after 3 months. Results obtained with the global activator phorbol myristate acetate, as well as with fMLF in TNF alpha-primed leukocytes, suggested that the deficiency of oxygen radical production in patients with severe GBS was the result of a specific deficiency of radical production in response to FPR/FPRL1 ligands rather than an inherent deficiency of NADPH oxidase function.

MEDICIN ->
Mikrobiologi, immunologi, infektionssjukdomar

Adult, Aged, Aged, 80 and over, Female, Guillain-Barre Syndrome, metabolism, pathology, physiopathology, Humans, Leukocytes, drug effects, metabolism, Male, Middle Aged, N-Formylmethionine Leucyl-Phenylalanine, analogs & derivatives, pharmacology, NADPH Oxidase, metabolism, Oligopeptides, pharmacology, Reactive Oxygen Species, metabolism, Severity of Illness Index, Tetradecanoylphorbol Acetate, pharmacology, Time Factors, Tumor Necrosis Factor-alpha, pharmacology

64427

2008-01-04 12:00

2010-01-26 12:48